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Next, muscles in all groups (except control) were made anoxic by replacing 95% Ocross-sectional area.

The average weight of trabeculae (n = 132) used was 3.11 ± 2.8 mg.

(-cyclohexyladenosine (CHA), 1% halothane, or 1.2% isoflurane in the presence and absence of 0.3 μm glibenclamide or 10 nm (APc DPCPX, isoflurane DPCPX) or 100 nm (CHA DPCPX) DPCPX for 5 min.

Time points corresponding to data reported in figures 3 and 4are denoted by arrows.(fig.

THE intraoperative and perioperative period is a time during which ischemia may be induced in patients by stress 1,2 and also by certain anesthetics.

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Appendages were transported from the operating room to the laboratory in oxygenated solution containing 130 mm Na Cl, 5 mm Na, 20 mm glucose, and 5 mm HEPES, and were oxygenated for a further 30 min.

The vapor concentrations used resulted in aqueous concentrations of 0.23 mm halothane and 0.26 mm isoflurane, which approximates the aqueous concentration at 37°C achieved by 1 minimum alveolar concentration of halothane (0.75%) or isoflurane (1.3%).

Anesthetic treatments were also performed in the presence of 0.3 μm glibenclamide and 10 nm DPCPX (fig. Muscles that served as time control or those subjected only to the subsequent anoxic insult alone (nonpreconditioned) received no treatment during this initial 5-min interval (fig. At the end of the treatment (time 5 min) and after a washout, a 15-min interval followed during which no treatments were administered.

The purpose of the current investigation was to determine any interaction between volatile anesthetics and preconditioning in a human model of simulated myocardial ischemia using anoxia (anoxic preconditioning).

Human right atrial appendages were acquired from patients undergoing coronary artery bypass grafting surgery before bypass in accordance with guidelines of the University Human Investigation Committee.